Fig. 7

Fig. 7

Vegf signaling upregulates etv2 expression. In situ hybridization analysis showing a decrease or increase in etv2 expression upon Vegfr inhibition or vegfaa overexpression, respectively. (A-D) Chemical inhibition of Vegfr with either SU5416 (A,B) or PTK787 (C,D) treatment starting at the 50% epiboly stage results in a decrease in etv2 expression when analyzed at 24 hpf. (E,F) vegfaa RNA overexpression results in an expansion of etv2 expression. Lateral view of 24 hpf embryos, anterior to the left. Numbers of embryos showing the displayed phenotype are listed. (G) etv2 expression in embryos treated with SU5416 at the 50% epiboly stage and analyzed at 2–3-somite (11 hpf), 10-somite (14 hpf), and 24 hpf stages was quantified by qRT-PCR. Note that etv2 expression in control DMSO treated embryos is increased at the 10-somite and 24 hpf stages compared to the 2–3 somite stage and this upregulation is reduced in SU5416 treated embryos while its initial expression at the 2–3 somite stage is not affected. All expression values are shown relative to etv2 expression at 24 hpf in DMSO treated embryos. (H) Quantification of etv2 expression at 24 hpf by qPCR in PTK787 treated and vegfaa RNA injected embryos. ** denotes p<0.01; * denotes p<0.05, ±SEM is shown. Arrows indicate DA and arrowheads indicate PCV.