Fig. 6

Fig. 6

Analysis of arterial and venous markers in vegfaa- overexpressing embryos. (A-R) In situ hybridization analysis of multiple arterial and venous endothelial markers upon vegfaa₁₂₁ and vegfaa₁₆₅ RNA injection as compared to uninjected controls. Arterial markers cldn5b (A and B), deltaC (C and D) and notch3 (E and F), venous markers stab1l (G and H) and flt4 (I and J), and pan-endothelial marker cdh5 (Q and R) are all expanded upon vegfaa RNA injection. In contrast, venous dab2, lyve1b and mrc1a expression is reduced in vegfaa overexpressing embryos (K-P). Note that dab2 and lyve1b expression is mostly confined to the PCV while stab1l and flt4 display some arterial expression in wt embryos (G,I,K,M). Arrows indicate the DA and arrowheads indicate the PCV. Lateral view of 24 hpf embryos, anterior to the left. Numbers of embryos showing the displayed phenotype are listed. (S) qPCR analysis of arterial (cldn5b, aqp8a), venous (flt4, stab1l, dab2, lyve1b, mrc1a) and pan-endothelial (cdh5, etv2) markers in vegfaa and control mRFP overexpressing embryos as compared to uninjected controls (wt). Note the significant increase in arterial and pan-endothelial marker expression and decrease in venous dab2, lyve1b and mrc1a expression in vegfaa overexpressing embryos. *, p<0.05; ** p<0.01 as compared to wt embryos, ±SEM is shown.