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Fig. 4
High concentrations of SU5416 result in apoptosis in trunk vasculature at 24hpf and 20-somite stages. (A,B) Caspase 3 immunofluorescent staining of 24hpf embryos treated with either DMSO or 10 μM SU5416 starting at the 50% epiboly stage. Strong Caspase staining, indicating apoptosis, was observed within trunk and tail vasculature in the 10 μM SU5416-treated embryos (B) as compared with the DMSO-treated control embryos (A). (C-H) Immunofluorescent staining of apoptotic marker Caspase3 in 20-somite stage embryos treated with either DMSO or 20 μM SU5416. Embryos treated with SU5416 showed strong Caspase3 staining which co-localized with vascular fli1:GFP fluorescence (F-H) compared to DMSO-treated embryos which did not have Caspase staining in the trunk vascular region (C-E). (I-N) 15-somite stage embryos treated with SU5416 did not appear to have apoptosis in the trunk region (L-N) and had comparable Caspase3 staining to the DMSO-treated controls (I-K).
Reprinted from Developmental Biology, 424(2), Chetty, S.C., Rost, M.S., Enriquez, J.R., Schumacher, J.A., Baltrunaite, K., Rossi, A., Stainier, D.Y., Sumanas, S., Vegf Signaling Promotes Vascular Endothelial Differentiation by Modulating etv2 Expression, 147-161, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.