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Fig. S6
vegfaa mutants do not display apoptosis in trunk vasculature at 24hpf. (A-F) Double immunofluorescent staining of Caspase3 and mCherry in vegfaa mutants (in a kdrl:mCherry background). mCherry staining marks vasculature, while Caspase staining indicates cells undergoing apoptosis. vegfaa mutants did not show Caspase staining in the trunk vasculature (E) and were similar to their wild type siblings (B).
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Reprinted from Developmental Biology, 424(2), Chetty, S.C., Rost, M.S., Enriquez, J.R., Schumacher, J.A., Baltrunaite, K., Rossi, A., Stainier, D.Y., Sumanas, S., Vegf Signaling Promotes Vascular Endothelial Differentiation by Modulating etv2 Expression, 147-161, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.