ZFIN Image: Chetty et al., 2017, Fig. S5

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Figure Caption

Fig. S5

Transient fli1:etv2-2A-mCherry expression in the vasculature does not affect the number of vascular endothelial cells. (A-C) Confocal images of trunk vasculature of a fli1:GFP embryo injected with a fli1:etv2-2A-mCherry construct at 24 hpf. Note that mCherry is expressed in a mosaic fashion within the vasculature. (D-F) Confocal images of trunk axial vasculature in a control uninjected fli1:nGFP embryo (D) and a fli1:etv2-2A-mCherry injected embryo (E,F) at 24 hpf. (G) Quantification of vascular endothelial cell number in uninjected fli1:nGFP embryos (n=6) and fli1:nGFP embryos injected with fli1:etv2-2A-mCherry construct (n=8) at 24 hpf. No significant difference in endothelial cell number was observed between control and injected embryos. The cells were quantified in the axial vasculature in a two somite region using lmaris software.

Acknowledgments

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Reprinted from Developmental Biology, 424(2), Chetty, S.C., Rost, M.S., Enriquez, J.R., Schumacher, J.A., Baltrunaite, K., Rossi, A., Stainier, D.Y., Sumanas, S., Vegf Signaling Promotes Vascular Endothelial Differentiation by Modulating etv2 Expression, 147-161, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.