IMAGE

Fig. S4

ID
ZDB-IMAGE-170512-12
Source
Figures for Chetty et al., 2017
Image
Figure Caption

Fig. S4

Analysis of marker expression and apoptosis in 28hpf embryos treated with SU5416 beginning at the 23-somite stage (20.5 hpf). (A) qRT-PCR analysis shows a significant decrease of all vascular markers in SU5416-treated embryos compared to DMSO-treated controls at 28hpf, after commencing treatment at the 23-somite stage. (B-G) Double immunofluorescent staining of active Caspase3 and GFP at 28hpf in fli1:GFP embryos treated with either DMSO or 20µM SU5416 (beginning at the 23-somite stage). Note the absence of Caspase3 staining in the trunk region of both the DMSO- and SU5416-treated embryos (C,F), indicating a lack of apoptosis in the trunk vasculature.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 424(2), Chetty, S.C., Rost, M.S., Enriquez, J.R., Schumacher, J.A., Baltrunaite, K., Rossi, A., Stainier, D.Y., Sumanas, S., Vegf Signaling Promotes Vascular Endothelial Differentiation by Modulating etv2 Expression, 147-161, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.