ZFIN Image: Chetty et al., 2017, Fig. S2

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Figure Caption

Fig. S2

PTK787 chemical inhibition decreases arterial and venous marker expression and overall endothelial differentiation. (A-F) In situ hybridization analysis for arterial cldn5b and venous flt4 and stab1l expression at 24 hpf. Embryos were treated with 200 µM PTK787 beginning at the 50% epiboly stage. Note the downregulation of cldn5b and flt4 expression in PTK787 treated embryos. Arrows indicate DA and arrowheads indicate PCV. Numbers of embryos showing the displayed phenotype are listed. (G) qPCR analysis of arterial (c/dnSb), venous (flt4, stab 1 /, dab2) and pan-endothelial (cdh5, etv2) marker expression in PTK787 treated embryos at 24 hpf. Note that arterial marker, pan-endothelial marker and venous flt4 expression is reduced, while stab1l and dab2 expression is not changed significantly.** denotes p<0.01; * denotes p<0.05; NS, not significant. ± SEM is shown.

Acknowledgments

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Reprinted from Developmental Biology, 424(2), Chetty, S.C., Rost, M.S., Enriquez, J.R., Schumacher, J.A., Baltrunaite, K., Rossi, A., Stainier, D.Y., Sumanas, S., Vegf Signaling Promotes Vascular Endothelial Differentiation by Modulating etv2 Expression, 147-161, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.