Community Action Needed: Please respond to the NIH RFI
ZFIN ID: ZDB-IMAGE-170505-10
Figures for Hasegawa et al., 2017

Figure Caption/Comments:

Fig. 6 S1

Generation of Tg(hsp:il1b).

(A) Comparison of zebrafish Il1b and human IL-1β amino acid sequences. Arrows indicate the human IL-1β cleavage site (D116) and the estimated zebrafish Il1b cleavage site (T124). (B) ISH analysis of il1b expression in Tg(hsp70l:il1b) at 6 hr after heat shock (HS). HS induced systemic il1b expression. Scale bar, 250 μm. (C) Absence of mCherry induction by fin fold amputation in the Tg(hsp70l:il1b). A stress caused by fin fold amputation did not induce mCherry expression. Scale bar, 100 μm. (D) TUNEL analysis of Tg(hsp70l:il1b) at 24 hpf after induction of il1b overexpression. The diagram depicts the experimental procedure. Scale bar, 500 μm.

Figure Data:
Acknowledgments:
ZFIN wishes to thank the journal for permission to reproduce figures from this article. Please note that this material may be protected by copyright.