Fig. 6
Reduced carotenoid concentration in plin6 knockout zebrafish.
Brightfield images of the flank and isolated scales from adult wild-type (A, C) and plin6 mutants (B, D) show a reduction in yellow carotenoid pigmentation in mutant tissue (red arrows in C, D). (E) Extraction of carotenoid pigment from wild-type and plin6 mutant integument (inset) and the corresponding absorbances of carotenoids from a β-carotene standard, wild-type skin (WT), and plin6 mutant skin (KO). (F) Quantification of skin carotenoid levels from wild-type (WT) and plin6 mutant skin (KO). N = 4; *** = p<0.001. (G) Quantification of skin triacylglycerol levels from wild-type (WT) and plin6 mutant skin carotenoid droplets (KO). N = 3; ** = p<0.02.