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Fig. S5
Müller Glia are absent in aggregates treated with 25μM DAPT.
Zebrafish retinal cells were cultured using the GFAP:GFP transgenic line which strongly labels Müller Glia (but is also expressed in undifferentiated cells at low levels). Aggregates were treated with either DMSO or 25μM DAPT from the equivalent of 45hpf onwards. (A-B) Aggregates treated with DMSO as a control. (A) GFAP:GFP expressing MG can be seen throughout the aggregate, extending axonal like projections into the aggregate (filled arrow). (B) DAPI. (C-D) Aggregates treated with 25μM DAPT. (C) GFAP:GFP can only be seen at low levels, and no axonal projections can be seen indicating a lack of MG present. (D) DAPI. Scale bar = 10μm.