ZFIN Image: Li et al., 2009, Fig. 5

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Figure Caption

Fig. 5

Behavior of vasa-depleted PGCs in vitro. MOvc and MOvas were zygotically injected at 1.5 ng/embryo and the resulting early NgVg gastrula embryos were dissociated for culture. (A–F) MOvas injection does not affect cell growth. Red fluorescence comes from a co-injected tracing dye. (E and F) MOvas injection does not affect PGC (green) formation. (G) Proliferation and motility of PGCs (arrow) at 1 dpc. At this stage both PGCs and somatic cells (asterisk) steadily change their shape, form filopodia and undergo ameba-like movements. The displacement of two PGCs on the merged image (left) reflects quick changes in cell shape during photography. (H) Serial micrographs showing the motility of PGCs at 3 dpf. (I) Time course of PGC numbers. Shown are six representatives of MOvc- and MOvas-injected embryos each.

Acknowledgments

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Reprinted from Mechanisms of Development, 126(5-6), Li, M., Hong, N., Xu, H., Yi, M., Li, C., Gui, J., Hong, Y., Medaka vasa is required for migration but not survival of primordial germ cells, 366–381, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.