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Fig. 3
Long term in vivo two-photon imaging of podocyte injury.
The pictures of panel a show that a subset of podocytes (arrowheads) in Chet larvae at 4 dpf only express eGFP and not NTR-mCherry and therefore are not vulnerable to MTZ treatment (representative image of n?=?3 experiments, scale bar represents 25??m). Panel b shows the morphological changes in the glomerulus during treatment with 5?mM MTZ as seen in the 3D reconstructions of long term 2-PM of Chet larvae beginning at 4 dpf. After approximately 5?hours, dilation of Bowman?s space occurred which decreased in the following time as shown by the double arrows at t?=?5:40, 8:00, 12:20?hours (scale bar represents 25??m). Panel c shows single frames of the detachment of two adjacent podocytes (arrowheads) between t?=?9 and 10?hours (asterisks indicate a capillary loop which is covered by podocytes, scale bar represents 10??m). The time series of single frames over 8?hours in panel d shows retraction of major processes of a single podocyte during treatment with 5?mM MTZ (scale bar represents 10??m).