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Fig. S12
(a) Zebrafish frozen tissue sections were subjected to immunofluorescence assay using anti-EGFR antibody. Pharyngeal arch: white arrows; Esophagus: green arrow; EGFR: red; Nucleus; blue. Magnification: x50, Scale bar = 100 µm. (b) Paraffin-embedded zebrafish tissues were subjected to immunohistochemistry using anti-EGFR antibody. Scale bar = 100 µm. (c) Cell lysates from WT and evark10 mutant embryos at 5dpf were processed for immunoblot analysis using anti-EGFR and β-actin antibodies. β-actin was used as a loading control. Similar results were observed in two independent experiments.
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