ZFIN Image: Yun et al., 2017, Fig. S1

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Figure Caption

Fig. S1

(a and b) The G to A substitution in splicing acceptor site of exon 12 in REP1 genes induced zebrafish evanescence (eva^rk10) mutant having abnormal stop codon by aberrant splicing. (b) eva^rk10 mutant protein lacks the C-terminal GDI domain of the wild-type REP1 protein of zebrafish. (c) Morphological phenotypes of WT and eva^rk10 mutant embryos were examined with dissecting microscope at 5 day post fertilization (dpf). Arrows indicate abnormal morphology of head, pharyngeal arch, swim bladder (black), and eye (red). (d) Apoptosis was visualized by TUNEL assay in WT and eva^rk10 mutant embryos at 5 dpf. (e) Paraffin-embedded zebrafish tissues were subjected to immunohistochemistry using anti-active caspase3 antibody. Eye: red arrow; Tectum: black arrow; Cerebellum: arrowhead Magnification: x50; scale bar = 100 µm. Similar results were observed in two independent experiments.

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Phenotype details

Acknowledgments

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