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Fig. 2
Specific labeling of fusion proteins in vivo. Spinning-disk confocal micrographs of live zebrafish embryos coexpressing mCherry/Y-FAST or mCherry/PYP labeled with 5 μM HMBR at 24 h postfertilization (HMBR channel: Ex/Em 491/525–539 nm; mCherry channel: Ex/Em 561/605–664 nm). (Scale bars, 200 μm.) Side-by-side images were recorded using the same settings.
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