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Fig. S3

ID: ZDB-IMAGE-170413-50
Publication: Zelina et al., 2014 - Signaling switch of the axon guidance receptor Robo3 during vertebrate evolution
All Figures: Figures for Zelina et al., 2014

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Fig. S3

Lack of high-affinity binding of hNetrin-1 to mammalian or nonmammalian Robo3 and action of a Src-family kinase and c-Abl inhibitor. after hNetrin-1 stimulation

(A) Human Netrin1-AP does not detectably bind COS cells expressing either mouse, zebrafish, chick Robo3A.1 while it binds its known receptor, human DCC, with high affinity. (B) Robo3Y1019F is normally expressed at the cell surface in transfected COS cells. (C) Lckl inhibits the tyrosine phosphorylation of endogenous Robo3 induced by Netrin- 1 in P19 cells. (D) The allosteric c-Abl inhibitor GNF2 does not inhibit tyrosine phosphorylation of endogenous Robo3 after Netrin-1 stimulation in P19 cells.

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