ZFIN Image: Mandal et al., 2017, Fig. 8

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Figure Caption

Fig. 8

Increased Wnt signaling promotes loss of the SHF. (A–C) Control sibling embryos and embryos after increased Wnt signaling at the SP stage. Increasing Wnt signaling prior to gastrulation leads to loss of the SHF-derived OFT smooth muscle (elnb). Inserts indicate embryos with two-color ISH for myl7 and elnb. Outlines indicate the hearts. (D) RT-qPCR for cardiac differentiation and SHF progenitor marker expression at 24 hpf. Increasing Wnt signaling prior to gastrulation promotes increased expression of the pan-cardiac differentiation marker myl7 and decreased expression of the SHF markers mef2cb and ltbp3 compared to WT sibling controls. Images are lateral views with anterior upward. Scale bar indicates 100 μm.

Acknowledgments

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Reprinted from Mechanisms of Development, 143, Mandal, A., Holowiecki, A., Song, Y.C., Waxman, J.S., Wnt signaling balances specification of the cardiac and pharyngeal muscle fields, 32-41, Copyright (2017) with permission from Elsevier. Full text @ Mech. Dev.