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Fig. S5
Xvelo Networks Consist of SDS-Resistant Backbones Attached to More SDS-Soluble Side Chains, Related to Figure 5
(A) Once incubated at a lower SDS concentration (0.5%), the side chains also are SDS resistant. Xvelo network self-assembly reactions were initiated by lowering the arginine concentration to 30mM (in 50mM HEPES, 100mM KCl [pH] 7.6) and 1 or 0.5% SDS was added to these networks. After 30 min incubation at room temperature, the networks were imaged with a spinning disc confocal microscope.
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Reprinted from Cell, 166, Boke, E., Ruer, M., Wühr, M., Coughlin, M., Lemaitre, R., Gygi, S.P., Alberti, S., Drechsel, D., Hyman, A.A., Mitchison, T.J., Amyloid-like Self-Assembly of a Cellular Compartment, 637-50, Copyright (2016) with permission from Elsevier. Full text @ Cell