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Fig. 4
Vsx1 and Olig2 are expressed by distinct subpopulations of NAPs in zebrafish developing hindbrain. (a) Quantification of non-apical mitoses expressing Vsx1:GFP and Olig2:GFP in hindbrain at 24, 36, 48 hpf. (b?b?) Single z-slice of a dorsal view of hindbrain at 36 hpf showing NAP in mitosis in SA position expressing Olig2:GFP (indicated by a dashed circle and white arrow). (c?c????) Single z-slice of a dorsal (c?c?) and transverse (c??,c????) views of hindbrain at 36 hpf, showing NAP in mitosis in SA position expressing Vsx1:GFP (indicated by a dashed circle and white arrow). (d?e?) Transverse hindbrain sections at 24 and 36 hpf showing NAPs in mitosis in VMB positions expressing Vsx1:GFP (indicated by a dashed circle and white arrow). (f) Quantification of NAPs in SA, VMB and DLB positions in the zebrafish hindbrain, expressing Olig2:GFP and Vsx1:GFP at 24, 36 and 48 hpf. Magenta (blue in (b?b??)) is PH3 staining and blue (red in (b?b??)) is Sytox nuclear counter stain. The grey and white dashed lines outline the basal and apical surfaces of the neuroepithelia, respectively.