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Fig. 5
Specificity of Tg(8xHs.NF?B:GFP,Luciferase) for NF-?B activity in zebrafish embryos. (A,B) 0.3?mM i?b?a morpholino was injected into transgenic embryos. Morphants at 3?dpf had increased expression of GFP in the skin (A) and proctodeum (B, arrows). GFP fluorescence is shown as a false-color heat map. The intensity of the laser was equal for both images to allow direct comparison of the GFP signal in morphants and controls. (C) Quantitative comparison of the GFP signal in the proctodeum of embryos injected with 0.3?mM standard control or i?b?a morpholino. Each dot represents an individual embryo at 2?dpf (mean±s.d.). (D) Injection of 1?mM i?b?a morpholino results in higher GFP expression and embryonic malformation. GFP fluorescence is shown as a heat map as indicated in A. Scale bars: 500?µm (A); 50?µm (B); 300?µm (D).