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Fig. 6
Rg1 supplementation impairs lipid accumulation in high fat diet-induced obese Zebrafish. (A) Lipid accumulation was evaluated by Nile Red staining in zebrafishes grown with HFD in the presence or absence of 5μM, 10μM, 2.5μM Rg1/5μM NAC during 20 dpf and visualized under a fluorescence microscope (n = 3). (B) Quantification of Nile Red fluorescence intensity. Results were analyzed by one-way ANOVA and Duncan's multiple range test. The experiment was performed in triplicate. Values with different superscript letters are significantly different, p < 0.05. (C) Triglyceride was subjected to TG assay in 20 dpf zebrafishes grown with HFD in the presence or absence of 5μM, 10μM, 2.5μM Rg1/5μM NAC, and measured at 540 nm. Results were analyzed by one-way ANOVA and Duncan's multiple range test. The experiment was performed in triplicate. Values with different superscript letters are significantly different, p < 0.05. ANOVA, analysis of variance; CON, control; dpf, days postfertilization; NAC, N-acetyl cystein; HFD, high fat diet; ND, nondifferentiated preadipocyte; TG, triglyceride.