ZFIN Image: Heanue et al., 2016, Fig. 2

Image

Figure Caption

Fig. 2

ret^hu2846/+ larvae exhibit ENS progenitor migration deficits.

ENS progenitor migration at the front of migration (wavefront) visualized in real time by virtue of the SAGFF234A;UAS:GFP background labeling ENCCs. (A) Representative still images from confocal time-lapse recordings (depth-coded to resolve 2 migratory streams) of WT (ret^+/+) and ret^hu2846/+ embryos at 0 minutes (48hpf) and 500 minutes (56hpf), show that distance travelled by the migration wavefront is decreased in ret^hu2846/+ larvae relative to WT larvae. Asterisk denotes a GFP+ cell from outside the ENS linage that remains in the same position. (B) The calculated wavefront migration speed (microns/minute) is significantly reduced in ret^hu2846/+ larvae relative to WT at 48–56hpf (p = 0.0022), and 72–80hpf (p = 0.0182).

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.