|
Fig. 3
Growth and caudal fin regrowth are maintained in the zWEDGI. (A) Images from a dissecting microscope showing larvae collected within 30 min of wounding (0 dpw), 1 and 2 dpw that were anesthetized and maintained in E3 buffer (unrestrained), 1% agarose or zWEDGI with 1% agarose over head region and tail unrestrained in buffer. Scale bar = 1 mm. (B) Graph showing body length (eye to edge of tail fin) in the three mounting conditions. (C) Graph comparing regrowth (regenerate length) of transected tail fin with the three mounting conditions. Data normalized to mean of 0 dpw for a given treatment, n ≥ 28 larvae per treatment over a total of three replicates. **p < 0.01, ***p < 0.0001. dpw, days post-wound.