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Fig. S5
Specificity of the anti-Vangl2 antibody.
(A-B) Dorsal view of wild type (A) and vangl2 mutant (B) 24 hpf neural tubes immunostained with anti-Vangl2-NT (green). The neuroepithelial membrane staining visible in wild type is absent in the mutant. (C) Western blot analysis of whole embryo lysates with anti-Vangl2 antibody. Anti-alpha-tubulin was used as a loading control. Zebrafish Vangl2 is expected to run at approximately 60kDa. For the anti-Vangl2 blot there is a band that is present in the wild type and absent in the vangl2 mutant, see asterisk.
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