Fig. 6
Chronic anemia model. Serial echocardiography was performed in male wild-type fish (n=8) to assess cardiac function before and after an 18-day phenylhydrazine hydrochloride (PHZ) treatment protocol to induce hemolytic anemia. (A) Representative explanted hearts from an untreated fish (left) and a fish treated with PHZ (right); treated hearts developed pallor and enlargement of the ventricle (v) and atrium (a). (B) Longitudinal axis images showing ventricular and atrial size before (left) and after (right) PHZ. Scale bar: 0.2 mm. (C-E) Description of bar graphs depicted in C-E as for Fig. 5. Data are shown as mean±s.e.m. *P≤0.05, **P≤0.01; ***P≤0.001 (paired t-test).