|
Fig. 4
Bone defects inwls,wnt9a,wnt5bandgpc4mutants. (A-J) Maximum intensity projections of live confocal imaging stacks of 4 dpf (A-E) and 8 dpf (F-J) Wnt homozygotes in Tg(sox10:GFP) background stained with alizarin red S. (K-O) 40X confocal maximum intensity projections of alizarin red stain focusing on the dentary bone (de) and mentomeckelian (mm). The mentomeckelian is seen as a flatter bone attached to the distal end of the dentary that is thicker. A clear boundary is observed between the mm and de. wls mutants lacks the dentary or mentomeckelian. (P-T) Confocal maximum intensity projections of osteocalcin transgene expression in Wnt mutants in a Tg(sox10:mCherry) background. (U-Y) Confocal maximum intensity projections of osx transgene expression in Wnt mutants in a Tg(sox10:GFP) background. (Z) Graphical map of neural-crest cell (NCC)-derived bone (red; intramembranous ossification and orange; endochondral ossification) and mesoderm derived bone (blue). Mentomeckelian (mm), Maxilla (mx), Dentary (de), retroarticular (ra), quadrate (q), ceratohyal (ch), branchiostegal rays (bsr), opercle (op), ceratobranchial 5 (cb5), cleithrum (cl). (AA) Percentage of mutant with bony phenotype of different bones in the craniofacial region where 100% indicates that all mutant embryos do not have that particular bone. Scale=50 µm.
Reprinted from Developmental Biology, 421(2), Ling, I.T., Rochard, L., Liao, E.C., Distinct requirements of wls, wnt9a, wnt5b and gpc4 in regulating chondrocyte maturation and timing of endochondral ossification, 219-232, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.