ZFIN Image: Wei et al., 2016, Fig. 2

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Fig. 2

Establishment of zfZNF32 knock-out zebrafish and lateral line system regeneration. A. Agarose gel electrophoresis showing the Cas9-mediated zfZNF^-/- with bases deleted compared with WT. B. A total of 85 bp were deleted (in blue font) in the zfZNF^-/-. C. The regenerative capacity of the lateral line system was increased in 4 dpf zfZNF^-/- at 12 and 24 hrs after neomycin treatment. Hair cells in the lateral line were stained with DASPEI (red fluorescence). The L1 was chosen as the representative regenerating neuromast, as indicated by arrows, and shown in the lower right corner of each figure with 200×magnification.

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Acknowledgments

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