|
Fig. 7
Figure 7 p53 inhibition abrogates the angiogenic defects caused by PEG-b-PCL nano-micelle.
Notes: (A) Representative images of flk1-GFP-positive vascular sprouting in 24 hpf control, Pifithrin-α pretreated and p53MO preinjected embryos with or without 240 μg/mL PEG-b-PCL exposure. White arrowheads indicate the absence of ISV and the dashed oval indicates the cavity in caudal vessels. (B) Quantification of ISV length as shown in (A). (C) In situ hybridization analysis of flk1 expression in 24 hpf control, Pifithrin-α pretreated and p53MO preinjected embryos with or without 240 μg/mL PEG-b-PCL exposure. Black arrowheads indicate the absence of flk1 expression. (D) qPCR analysis of flk1 mRNA expression in 24 hpf control, Pifithrin-α pretreated and p53MO preinjected embryos with or without 240 μg/mL PEG-b-PCL exposure. Data are expressed as mean ± SEM from three biological samples. *P<0.05, ***P<0.001.
Abbreviations: Ctrl, control; hpf, hours postfertilization; ISV, intersegmental vessel; MO, morpholino; PEG-b-PCL, poly(ethylene glycol)-b-poly(ε-caprolactone); qPCR, quantitative polymerase chain reaction; SEM, standard error of mean.