|
Fig. 5
Generation of a ttn.2/1 near-null mutant by targeting two highly used exons. (A) Schematic representation of the ttnxu071 mutant. Arrows indicate locations of mutations. (B) Analysis of protein extracts from 2?dpf WT larvae and ttn.2xu065, ttn.1xu066 and ttnxu071 mutants. (C) qPCR with primers targeting ttn.2 and ttn.1, respectively. Meansħs.d., N=5. *P<0.05, ** P<0.01. (D) The Z-disk structure marked by ?-Actinin was disrupted in the ttnxu071 mutant. (E) Electron micrographs of somites of 2?dpf WT and ttnxu071 mutant larvae. (F) Z-disk structure was normal in ttn.2xu065/+ and ttn.1xu066 at 2?dpf, but sarcomere disarray was noted in ttnxu071/+ and ttn.1xu066. Scale bars: 20??m (D,F) and 1?µm (E).