ZFIN Image: Miranda-Rodríguez et al., 2017, Fig. S4

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Figure Caption

Fig. S4

Inactivation of ROCK affects PGCs specification. (A, B, C and D) nanos mRNA in situ hybridization localization in 128 to 256-cell stage embryos. Arrowheads indicate the positive signal. Embryos at 128–256 cell stage normally have four cells that express mRNAs of the GP such as nanos (D), injected embryos with h1152 showed less positive cells as shown in (A, B and C). (E) The number of embryos with a normal number of positive cells for nanos was quantified in 128–256 cells embryos injected at one cell stage, either with control buffer (n=46) or with h1152 (n=63). The error bars represent the standard error of the mean (* p<0.05).

Acknowledgments

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Reprinted from Developmental Biology, 421(1), Miranda-Rodríguez, J.R., Salas-Vidal, E., Lomelí, H., Zurita, M., Schnabel, D., RhoA/ROCK pathway activity is essential for the correct localization of the germ plasm mRNAs in zebrafish embryos, 27-42, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.