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Fig. S15
Simultaneous siRNA knockdown of either cdkn1a or cdkn1c increases myocardial proliferation in dn-xBrg1 transgenic hearts. (a, b) Quantitative PCR showed that another independent siRNA for cdkn1a (a) or cdkn1c (b) decreased the RNA levels of cdkn1a and cdkn1c in wild-type hearts at 2 dpa. Control, cdkn1a 2# (a), or cdkn1c 2# (b) siRNA were injected at 1 dpa. The RNA level was normalized to GAPDH (**p <0.01, ***p < 0.001; data presented are mean ±s.e.m.; paired Student’s t-test). (c-f) BrdU+/Mef2C+ proliferating cardiomyocytes increased in dn-xBrg1 transgenic hearts at 14 dpa injected with either cdkn1a 2# (d) or cdkn1c 2# (e) compared with control NC siRNA (c). Statistics of panels c-e is shown (f) (*p <0.05, ***p <0.001; data are mean ± s.e.m.; one-way ANOVA followed by Dunnett’s Multiple Comparison Test; nc served as control). The number (n) of hearts analyzed in each group is indicated in each bar; heat shock was applied from 5 to 14 dpa. Scale bar, 100μm.