|
Fig. S1 Schematic representation of the Tol2-mediated GBT vector composed of a protein trap cassette and a 3’ exon trap cassette. Insertion of GBT vector (detailed in upper row) into intronic sequence of a gene disrupts native splicing of the gene. The artificial splice donor (SD) and splice acceptor (SA) in the GBT vector fuse with upstream SD and downstream SA, respectively. This results in truncation of the native protein (illustrated in bottom row) Abbreviations: ITR: inverted terminal repeat; SA, splice acceptor: poly(A): polyadenylation signal with extra transcriptional terminator; β-actin: carp beta-actin enhancer and promoter, with noncoding exon and intron 1 sequences; SD, splice donor; E, enhancer; P, promoter.