ZFIN Image: Jiménez-Amilburu et al., 2016, Fig. S2

Image

Figure Caption

Fig. S2

Apical constriction is recovered after restoring blood flow in larval zebrafish hearts

(A-B'') Confocal images (mid-sagittal sections) of 100 hpf Tg(-0.2myl7:EGFP-podxl);Tg(myl7:MKATE-CAAX) zebrafish hearts after 20 mM BDM treatment. Boxed area in A shown in A' and A''. Untreated larvae at 100 hpf showing depolarized cardiomyocytes (A' and A'', asterisks). Boxed area in B shown in B' and B''. Larvae were treated at 56 hpf and imaged at 100 hpf. BDM-treated larvae did not exhibit any apical constriction or depolarized cardiomyocytes (B' and B'', arrows). (C-E') Confocal images (mid-sagittal sections) of 79 hpf Tg(-0.2myl7:EGFPpodxl); Tg(myl7:MKATE-CAAX) zebrafish hearts after 20 mM BDM treatment. Boxed area in C shown in C' and C''. Untreated larvae at 79 hpf showing apical constriction (C' and C'', arrowheads) and depolarized cardiomyocytes (C' and C'', asterisks). Boxed area in D shown in D'. Larvae were treated at 72 hpf and imaged at 79 hpf. After 7 hours of treatment, larvae did not exhibit any apical constriction or depolarized cardiomyocyte (D', arrows). Boxed area in E shown in E'. At 79 hpf, BDM was removed and washed out, and larvae were then imaged at 96 hpf. Imaging reveals presence of apical constrictions (E', arrowheads). At, atrium; V, ventricle; AVC, atrioventricular canal. Scale bars, 20 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.