IMAGE

Fig. S1

ID
ZDB-IMAGE-161222-32
Antibodies
Source
Figures for Alvarado et al., 2016
Image
Figure Caption

Fig. S1

Detection of endogenous Wdr68 and ectopic GFP-Wdr68 fusion protein in zebrafish embryos.

A-D) Immunofluorescence detection of endogenous Wdr68. A) Wdr68 expression in a wildtype 15-somites stage embryo raised at 32°C. B) a wdr68 mutant lacking Wdr68 protein. C) Wdr68 expression in a wildtype prim-5 stage embryo raised at 32°C. D) a wdr68 mutant lacking Wdr68 protein. E-F) live imaging of GFP fluorescence in Tg(hsp70l:GFP-wdr68)csu9 animals. E) transgenic animal with the only detected GFP expression coming from the cmlc:eGFP marker for transgenesis. F) same animal as in E but after a 0.5 hour heat shock (HS) at 39°C followed by 7 hours of recovery at 28.5°C. G) western blot analysis of Tg(hsp70l:GFP-wdr68)csu9 animals after various lengths of heat shock exposure. Panel G1) GFP-wdr68 expression is induced by heat shock. Panel G2) β-tubulin expression was used as a loading control and did not differ substantially between lanes.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One