ZFIN Image: Greaney et al., 2017, Fig. 4

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Figure Caption

Fig. 4

Birthdating Analysis by photoconverted fluorescent Protein Tracing In vivo, with Subpopulation Markers (BAPTISM) method (Caron et al., 2008) for identifying the time of terminal differentiation (birthdate) of motoneurons in Tg(huC:Kaede; isl1:GFP) zebrafish, which express photolabile fluorescent protein Kaede pan-neuronally. (A) Neurons born by the time of initial photoconversion (one of five timepoints shown) contain converted Kaede in initial image, taken at 5 dpf. Second photoconversion converts remaining Kaede, leaving GFP as the only green signal in Final image. Images are compared to determine birthdate of GFP+ motoneurons. (B) Dorsal plane (z12, dashed line) from a 5 dpf Tg(huC:Kaede; isl1:GFP) larva initially photoconverted at 36 hpf. Yellow arrowheads indicate an nIII motoneuron (isl:GFP+, final image) born by 36 hpf (converted huC:Kaede+, initial image). White arrowheads indicate a neuron born by 36 hpf not belonging to nIII/nIV. Orange arrowheads indicate an nIV motoneuron not born by 36 hpf. Scale bars = 20 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Comp. Neurol.