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Fig. S1
Treatment with VEGFR inhibitor during acute ethanol exposure did not alter the cellular responses of hepatic stellate cells (HSCs) to ethanol. (A) Work flow of the ethanol and VEGF receptor (VEGFR) inhibitor co-treatment experiment. (B-E) Confocal single plane images of Tg(hand2:EGFP;kdrl:ras-mCherry) larvae treated with 0.03% DMSO (A), 2% ethanol and DMSO (B), 3 μM ZM306416 (ZM) (C), 2% ethanol and 3 μM ZM (D) from 96 to 120 hpf. The HSCs are labeled by Tg(hand2:EGFP) expression (green), and the endothelial cells are marked by Tg(kdrl:ras-mCherry) expression (red). Ventral view, anterior is to the top. Scale bar, 30 μm. (F) Numbers (mean±s.e.m.) of HSCs per liver in each treatment group. Three separate experiments were performed and the total numbers of animals analyzed are shown. One-way ANOVA and Tukey’s post-hoc test indicated that there is no statistical difference among each group (p>0.05).