Fig. S2
Inhibition of N-glycosylation shows no effect on the responsiveness to TGF-beta agonist Squint. (A) Classification of morphological phenotypes of WT embryos injected with squint mRNA. WT embryos were injected with 10 pg of squint mRNA at 1-cell stage, treated without or with 50 μg/ml SW from 4 to 24 hpf and then analyzed for morphological phenotypes. cI–cIII, class I–class III. cIV embryos were dead from over-involution at the time of analysis. (B) Percentage of embryos with different classes of phenotypes. The number of embryos analyzed is as indicated. The difference between the control and SW treated groups is not significant (n. s., p = 0.33).