Fig. S3

Fig. S3

Estimated size for cytoplasmic granules. (A) Graph comparing average area (µm²) for P54a-EGFP and P54b-EGFP cytoplasmic granules. Area values were estimated with ImageJ and later converted to diameter values considering that granules have a circular shape. Under normal temperature P54a-EGFP granules have an average area of 0.07 m2 and a diameter of 0.30 µm², while P54b-EGFP granules have an average area 0.06 µm² and a diameter of 0.29 µm². In heat shock conditions, the average area and diameter for P54a-EGFP cytoplasmic granules was of 2.0 µm² and 1.63 µm², respectively. For P54b-EGFP granules, under heat shock the average area and diameter for cytoplasmic granules was of 0.8 µm² and 1.03 µm², respectively. (B) Example of P54a-EGFP granules in normal conditions used in our measurements. (C) Example of P54a-EGFP granules in heat shock. (D) P54b-EGFP granules in normal conditions. (E) P54b-EGFP granules in heat shock.