ZFIN Image: Keßler et al., 2015, Fig. 5

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Fig. 5

Reduced ATP levels in ste^−/− mutants lead to sinus bradycardia. a ATP content of ste^−/− mutant embryos is significantly reduced compared to wild-type embryos (TE strain) and wild-type ste siblings (ste^+/−;+/+), whereas ATP content between TE strain and wild-type ste siblings does not significantly differ. Dead zebrafish embryos serve as a control (72 hpf). The ATP content of embryos that were incubated with SFA and DNP is also significantly reduced and resembles the ATP content of ste^−/− mutant embryos. b The coenzyme Q10 analogue Idebenone partially rescues heart rate in ste^−/− mutant embryos that were incubated with this drug. cste^−/− mutants were incubated at increased temperature throughout embryonic development and ventricular fractional shortening was measured in wild-type and ste^−/− mutant embryos at 72, 96 and 120 hpf. Fractional shortening of ste^−/− mutant embryos under normal conditions (29 °C) does not significantly differ compared to wild-type embryos. Fractional shortening is reduced in ste^−/− mutant embryos raised at 32 °C at (48 ± 2.1 vs. 54 ± 3.9 %, n = 10, p = 0.0358), 96 hpf (18 ± 4.0 vs. 56 ± 2.0 %, n = 10, p < 0.0001) and 120 hpf (10 ± 3.5 vs. 56 ± 2.1 %, n = 10, p < 0.0001)

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Basic Res. Cardiol.