|
Fig. S2
RT-PCR expression analysis of slc7a6os embryonic and adult zebrafish.
(A) Real-Time PCR expression analysis of slc7a6os throughout Danio rerio development. All reactions were run in triplicate. The relative expression levels, represented as the meanąSEM in log2 scale, were determined with respect to the 1-cell stage and normalized to elongation factor 1? (ef1?). (B) RT-PCR expression analysis of slc7a6os in adult zebrafish tissues. Beta-actin was also amplified as housekeeping gene internal control. 1: brain; 2: intestine; 3: eye; 4: heart; 5: kidney; 6: swim bladder; 7: branchias; 8: testis; 9: ovary; 10: negative control.