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Fig. 2
Cadherin-2 is required cell autonomously for caudal migration of FBMNs.
(A-I) Whole-mount immunocytochemistry showing dorsal views of Tg(isl1:GFP) (A-C) and Tg(isl1:cdh2ΔEC-mCherry)vc25 transgenic embryos (D-I) at 38 hpf embryos. Embryos are labeled with α-GFP (green) (A,D,G) and α-mCherry (red) (B,E,H) antibodies. (A-C) Wild-type Tg(isl1:GFP) embryos with FBMNs fully migrated into r6. (D-I) Defective caudal migration of FBMNs in Tg(isl1:GFP)/Tg(isl1:cdh2ΔEC-mCherry)vc25 embryos carrying one copy of the transgene (hemizygous) or two copies (homozygous). (J) Histograms indicate the percent of FBMNS at 38 hpf that failed to migrate (r4), migrated partially (r5), or migrated fully (r6). Each histogram corresponds to the genetic condition in the image to its left and numbers indicate the number of FBMNs counted. White asterisk denotes r6-derived PLL efferent neurons, which differ from r4-derived FBMN populations.