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Fig. 1
GFP is highly expressed in the ventricle of v12 zebrafish embryos. (A-D?) GFP is not detected in 75% epiboly embryos (A,A?), and is initially expressed in the somite boundaries at ~24?hpf (B). Cardiac GFP expression starts at ~35?hpf (C,C?) and GFP is highly expressed in the ventricle at 50?hpf (D,D'). Arrows indicate ventricle. (E-H) Ventricular GFP expression in v12 heterozygotes (E-F) and v12 homozygotes (G-H) at 84?hpf shown at high magnification. Arrows indicate ventricle. (I-I'') Immunostaining shows that ?-catenin and GFP colocalize close to the cell membrane in v12 heterozygous hearts at 78?hpf. (J-J'') Immunostaining shows that GFP is absent from Z-disc (?-actinin) in v12 heterozygous hearts at 78?hpf. Scale bars: 100?µm in A-D,E-H; 20?µm in I; 5?µm in J.