Fig. S4
Expression of Vegf family members in zebrafish CMs. (A) FACS isolation of EGFP+ CMs from uninjured Tg(myl7:EGFP) adult zebrafish hearts. Cellular debris were excluded, and the gated population of EGFP+/propidium iodide (PI)- cells was sorted. (B) qPCR analysis of myl7 (as a positive control), vegfaa, vegfab, vegfc, vegfr1, vegfr2, vegfr3, and six3b (as a negative control) expression in FACS-sorted myl7:EGFP+ CMs. (C) qPCR analysis of vegfab expression at 6 hpi and 1, 2, 3, and 5 dpi. Data (mean ± SD) are shown relative to their respective sham controls. *P < 0.05 (n = 4, each replicate consists of a pool of 4 ventricles).