Fig. 4
Autophagy as an energy-providing and recycling process. (A to H) Fed and fasted fish were myectomized and treated with or without CQ. Proliferation was visualized at 24 hpi staining for EdU; the inset shows the differential interference contrast image. (I) Quantification of proliferation showed that both fasting and CQ treatment reduced proliferation in the regenerating LR muscle; values are averages ± SEM (Student t test, *, P < 0.05; **, P < 0.01; n = 4). (J) To better illustrate the interaction of feeding condition and CQ treatment, proliferation values were normalized to the 0 mM CQ value of each group showing that CQ treatment reduced proliferation similarly in both fed and fasted fish. Values are averages ± SEM (Student t test; *, P < 0.05, n = 4).