IMAGE

Fig. 6

ID
ZDB-IMAGE-161103-44
Source
Figures for Perez-Camps et al., 2016
Image
Figure Caption

Fig. 6

Sox32 modulates Oct4 and Nanog complexes in ventrolateral endoderm of gastrula embryos.

(a) The schematic shows the Ventrolateral (VL)- and dorsal (D)-mesendoderm layers at 60% epiboly (7 hpf), with mesoderm in orange and endoderm in yellow. (b, c) Lifetime values (b) and FLIM images (c) of GFP-Oct4 alone and in the presence of mCherry-Nanog in the nuclei of individual cells within VL-Mesoderm and VL-Endoderm cells of wt and elabr13 mutants. Values of FLIM data represent the median and quartile ranges of data from three to five independent experiments (= 20–40 cell nuclei from 10 embryos; ***p<0.0001). Scale bar: 5 µm. (d) qRT-PCR analysis of sox32 relative to actin in wt and elabr13 mutant embryos. Values represent mean ± SEM of data from three independent experiments (**p<0.01). (e) Graphs show percentage of binding of GFP-Oct4 and mCherry-Nanog in VL-Mesoderm and VL-Endoderm of wt and elabr13 mutant embryos. Values represent the median and quartile ranges from data of three to five independent experiments (= 20-40 cell nuclei from 7 to 10 embryos; **p<0.01). Values represent mean ± SEM of data from three independent experiments (**p<0.01). n.s. over bars indicates non-significant differences. (f) qRT-PCR analysis relative to actin reveals different transcription levels of bmp2b, bmp4, bmp2a and her5 in elabr13 mutants at 60% epiboly (7 hpf). Values represent mean ± SEM of data from three independent experiments (**p<0.01). (g) bmp4 expression (top view, dorsal is to the right-hand side) is ventrally reduced in elabr13 mutants compared with wt embryos. her5 expression (dorsal view) is dorsally upregulated in elabr13 mutants related to wt embryos. See also Figure 6-figure supplement 1.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife