ZFIN Image: Perez-Camps et al., 2016, Fig. 2 S2

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Figure Caption

Fig. 2 S2

Dextran red does not interfere in the FCS measurements.

(a) Embryo (oblong stage; 3.5 hpf) showing mesendoderm (ME) labeled by dextran (red) leaving the ectoderm (EC) unlabeled. Scale bar: 200 µm. (b) ACF of the intensity traces of GFP-Oct4 in ME cells labeled or unlabeled. FCS data were fit by two-component anomalous diffusion model. (c) Raw data of residuals from fit curves shown in b. (d) DNA-bound fraction and diffusion coefficient derived from the FCS measurements in b. Values represent mean ± SEM of data from three independent experiments (n = 40-100 cell nuclei from 10 to 15 embryos). n.s. over bars indicates non-significant differences.

Acknowledgments

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