ZFIN Image: Nissim et al., 2016, Fig. S2

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Figure Caption

Fig. S2

nr5a2 mutants and morphants display normal cell death and proliferation programs. A) TUNEL staining in nr5a2^+/+, nr5a2^+/oz3, and nr5a2^oz3/oz3 mutant embryos at 36 - 48 hpf. nr5a2^oz3/oz3 mutants do not show indications of excess cell death relative to nr5a2^+/+ sibling controls. B) TUNEL staining in nr5a2 morphant embryos at 60 - 84 hpf. nr5a2 morphants do not show indications of excess cell death relative to controls, demonstrating that cell death is not a likely cause of liver or exocrine pancreas reduction. C) TUNEL staining in nr5a2 morphant embryos at 84 hpf. nr5a2 morphants do not show indications of excess cell death relative to controls, demonstrating that cell death is not a likely cause of exocrine pancreas reduction. D) Immunohistochemistry for phospho-histone 3 (PH3) at 24 hpf. nr5a2^oz3/oz3 embryos have no indication of reduced cellular proliferation in the endoderm region.

Acknowledgments

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Reprinted from Developmental Biology, 418(1), Nissim, S., Weeksb, O., Talbot, J.C., Hedgepeth, J.W., Wucherpfennig, J., Schatzman-Bone, S., Swinburne, I., Cortes, M., Alexa, K., Megason, S., North, T.E., Amacher, S.L., Goessling, W., Iterative use of nuclear receptor Nr5a2 regulates multiple stages of liver and pancreas development, 108-23, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.