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Fig. 5
Nuclear progestin receptor is up-regulated by estrogen in both the developing and adult brain.
Fold induction of pgr expression after treatment of adult zebrafish with an aromatase inhibitor (A, 10-6 M of ATD) or estradiol (B, 10-7 M of 17β-estradiol), and larvae with E2 (C, 10-8 M of 17β-estradiol). In A, the aromatase inhibitor (ATD) leads to a significant decrease of pgr expression in individual brains of adult zebrafish (n = 4). In B, the estrogenic treatment leads to a significant increase of pgr expression in pools of 5 brains of adult zebrafish (n = 3). In C, the estrogenic treatment leads to a significant increase of pgr expression in pool of 20 larvae (n = 2). Asterisk (*) indicates significant differences (p<0.05, Student′s t test). The graphs present the mean value +/- the standard deviation.