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Fig. 1
Pluripotency Timing during Embryogenesis in Zebrafish
(A) Representative morphology of early embryos at 256-cell, high, oblong, sphere, dome, 30%-epiboly, 50%-epiboly, and 70%-epiboly stages. hpf, hours post fertilization. Scale bar, 250 µm.
(B) Morphology of isolated embryonic cells from different stages. Asymmetric spread of cytoplasm was observed in all stages (lower panel). Scale bar, 50 µm.
(C) RT-PCR analysis of gene expression patterns in different stages of the embryos. Primer sequences and PCR conditions are listed in Table S1. NTC, no template control.
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