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Fig. S1
Related to Figures 1 and S. Quantitative Analysis of Be Morphology. (A) The stratification levels of Be axons were analyzed by measuring the distance of the axon terminal from the inner (0% IPL width) to outer IPL (100% IPL width) , and normalizing to the total width of the IPL. The IPL was visualized by an actin or nuclear stain. (B) The locations of Be somata within the INL were assessed by measuring the distance from the center of the soma to the OPL, and normalizing to the total width of the INL. The INL width was judged as the distance from the outer boundary of the IPL (0% INL width) to the OPL (100% INL width). (C) The sizes of Be dendritic territories were analyzed by visualizing a maximum intensity projection of the dendritic arbor en face, drawing a boundary around the outermost dendritic tips, and measuring the area within the resulting polygon (shown outlined in cyan). (D) Be axon terminal size was quantified as the area of the axon terminal in a thresholded maximum intensity projection. This was achieved by converting the image fluorescence into a binary mask, and measuring the masked area that corresponded to the individual axon terminal (shown outlined in cyan).